Part:BBa_K563004:Experience

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•••• UCSF-UCB iGEM 2014

We used pTEF1 in pSV606 (S. cerevisiae) yeast strains to create a constitutive circuit for our project, and it performed consistently as a constitutive promoter. We characterized pTEF1 alongside some of its mutants (m3, m6, m7, and m10), as seen in the following graph. If one would like to optimize expressiveness, pTEFm7 was found to have a higher level of constitutiveness than pTEF1, but pTEF1 is still a reliable promoter. The mutants were selected from a 2006 paper by Elke Nevoigt and Jessica Kohnke: http://www.ncbi.nlm.nih.gov/pubmed/16885275. pTEF1 is also listed as parts BBa_K431008 and BBa_K319003.

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•••• Team Chalmers Gothenburg 2015

Summary: The high expression pTEF1 promoter connected to a truncated pSUC2 promoter. Improves the function of pTEF1 by adding repression at high levels of ATP and induction at low levels of ATP. For more info BBa_K1603001